Neil Critchley's young side bounced back to winning ways on Sunday with a 4-0 success over Stoke City at St George's Park, and will be full of confidence ahead of this tie.
Porto's B team are the current holders of the competition and they play their domestic football in a senior league.
Read on for more details ahead of Wednesday night's encounter...
Where is the game being played? Due to severe weather conditions on Merseyside, the match is now taking place at Leigh Sports Village with kick-off set for 7pm GMT. There will be no admittance for supporters due to the game being classified as a behind closed doors fixture at the request of the Premier League.
How can I watch the game? The game is live on LFCTV and LFCTV GO, with our coverage starting at 6.30pm. Click here to sign up now.
View from the manager, Neil Critchley
"The reason why we entered this competition is to get the chance to play teams like Porto. They won this competition last year. They have a very experienced senior B team so it’s a slightly different outlook and approach they have in Portugal, so it’s a game that we know we are going to have to play very well in to get something out of. The way our boys are playing at the moment I would back them against anyone, so it’s a great game for us to play and I’m really looking forward to the challenge."